FORMULATIONS
 
 

Physiological Salines: These solutions are formulated to simulate the ionicity - concentration of salts - of various blood and other plasmas, lymphatic fluid, and cellular exudations.

Simplified Amphibian Ringers

  Sigma MWt g1^-1 For 3 litres

113.0 mM NaCl

 S 5886 58.44 6.600 g l^-1 19.8
1.0 mM CaCl2 C 7902 147.00 0.147 g l^-1 0.441
2.0 mM KCl P 5405 74.55 0.149 g l^-1 0.447
3.6 mM NaHCO3 S 5761 84.01 0.302 g l^-1 0.906

 

Modified Amphibian Ringer

  Sigma MWt g1^-1 For 3 litres

100.0 mM NaCl

 S 5886 58.44 5.840 g l^-1 17.52g
2.0 mM CaCl2 C 7902 147.00 0.294 g l^-1 0.882g
1.8 mM KCl P 5405 74.55 0.134 g l^-1 0.402g
1.0 mM MgCl2 M 2393 203.30 0.203g1^-1 0.609 g
5.0 mM Na-Hepes H 3784 260.30 1.300 g1^-1 3.900g

 

Phosphate Buffered Water

Phosphate buffer usually is buffered to pH 7.2. A convenient site for the calculation of the amounts of compounds for phosphate buffers of different pH and strength may be found at http://members.nuvox.net/~on.jwclymer/phos2.html.

Or a simple formula is (as table below) for a buffer of pH 7.2 is 35ml of 0.2M NaOH plus 50ml of 0.2M NaH2PO4.

0.2M NaOH = (MW= 40) therefore 0.2M is 8g per. liter.
0.2M KH2PO4 = (MW = 120) therefore 0.2 M is 25g per. liter.

This table shows the volume (ml) of 0.2M HaOH solution to be added to 50ml of 0.2M KH2PO4 to achieve a particular pH.

pH
NaOH (mL)
pH
NaOH (mL)

5.8
3.7
7.0
29.6
6.0
5.7
7.2
35.0
6.2
8.6
7.4
39.5
6.4
12.6
7.6
42.8
6.6
17.8
7.8
45.2
6.8
23.7
8.0
46.8

 

 

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