Luteinizing Hormone Releasing Hormone analogue (LHRHa) Sigma L4513

NB: Of the different types of LHRHa available this is the only one that has been found effective with toads. This hormone is an analogue of gonadotrophin releasing hormone (GnRH) which is released by the hypothalamus and controls release of the gonadotrophic hormones follicle releasing hormone (FSH) and luteinising hormone (LH).

This hormone comes as a powder, which must be resuspended before administration by injection. This hormone is effective at very low and uncertain doses of between 4-10 micrograms (µg). The dose of LHRH we have used with Wyoming toads (Bufo baxteri) in combination with hCG at 500IU is 4 µg of LHRHa per 50 gram toad. A milligram (mg) is one thousandth of a gram (1 x 10^-3g), a microgram is one millionth of a gram (1 x 10^-6g), and a picogram (pg) is one billionth of a gram (1 x 10^-9g). The dose of LHRHa we have used with Wyoming toads (Bufo baxteri) in combination with hCG at 500IU is 4 µg. The vials from Sigma come in 1 mg amounts. Dilute the LHRHa in the vial with 5 ml of sterile water; resulting in a concentration of 0.2 mg/ml and a dose of 4 µg per. 20 µl. This stock solution can then be stored indefinitely in Eppendorf tubes at -80°C in 100 µl aliquots, which will give five doses of 4 µg. If the LHRHa you order is of a different amount per. vial, or the doses you want to administer differ from 4 µg you will need to adjust the values above accordingly.