Excised Testis

The left and right testes as excised. The left testes in two pieces and these approximate the right testes in size. The testes when first removed have blood and other tissue attached. When removed the testes should be cleaned of blood. They should then be macerated in Simplified Amphibian Ringers at an amount of one part of testes to one part SAR 1:1 (w/v), and this macerate or the whole testes stored in Eppendorf tubes in ice slurry. Whole testes do not store well and it is preferable to make testes macerates as soon as possible.